Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZNF335 in samples. An antibody specific for ZNF335 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZNF335 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF335 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF335 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: ZNF335 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure.
The deduced 1,342-amino acid NIF1 protein contains 6 predicted C2H2 zinc fingers, of which the first 3 comprise a BED finger DNA-binding domain; an LXXLL motif; a C-terminal leucine zipper region; an N-terminal 35-amino acid region enriched in acidic residues; and putative protein kinase A and tyrosine kinase phosphorylation sites. NIF1 acts as a regulator or cotransducer of NCOA6 coactivator function